Bio 1406 Enzyme Report

1) ABSTRACT The purpose of this sample was to carry forth the set go forth of enzyme concentration, temperature, pH level, and an inhibitor on a type of enzyme called Perixodase. The purpose of the prove was also to understand the relationship between the change in absorbance with time and the enzyme response direct. To perform the experiment, the class was divided into groups. sepa regularizely group took on one of the factors affecting the enzyme. Before hold the actual experiment, we apply the mixing tables that were given to us to pose up the test tubes with the appropriate substances. We followed the same procedure for each experiment variable (1-5). We then rid the cell of toxic hydrogen peroxide (H2O2) so the cell would not train damaged, as stated in our lab manual. For every experimental variable, test tube 1 was always the control. Once the solutions were trip out we mixed them and poured them into the cuvette and employ the spectrophotometer ma chine to run the enzyme and determine its rate and its absorbance. When the actual experiment was done we all shared the results and graphed them. 2) mental hospital Before beginning the actual experiment we had to become well-known(prenominal) with a spectrometer machine and its functions.

It is basically an instrument used to measure the amount of light absorbed by a solution (Frankhauser, David B). As stated in our lab manual, the darker the solution, the great its absorbance. The reason why we used this machine was to figure out the relationship between the change in absorbance with time and the enzyme reaction rate. The enzyme reaction rate was determined when plot of la nds of absorbance versus time were construct! ed. The slope of all(prenominal) plot was related to the reaction rate. After running a nonaged experiment to learn how to use the spectrophotometer, we learned or so the background information of enzymes. Enzymes are proteins that serve as catalyst (Ophardt, Charles E.). By definition, a catalyst is a substance that initiates or accelerates...If you want to get a full essay, order it on our website: OrderEssay.net

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